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Puromycin-based vectors promote a ROS-dependent recruitment of PML to nuclear inclusions enriched with HSP70 and Proteasomes

At 25M of menadione, there was a 13.3-fold increase for HEK-293 cells and 11.5-fold increase for K-562 cells in the amount of oxidized glutathione compared to the control

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Yes, and this is a practical protocol concern

This work was partly supported by the Tohoku Medical Megabank Project from MEXT, Japan Agency for Medical Research and Development (AMED